Open Seminars of the Research Group on

 

Stochasticity and Control in the Dynamics and Diversity of Immune Repertories: an Example of Multi-Cellular Co-Operation

 

Tuesday, May 9, 2017, 14:00

at the Israel Institute for Advanced Studies, Room 128

 

 

Deciphering the family tree of immune and blood cells

 

Leïla Perié (Institut Curie)

 

How heterogeneous systems of cells constituting multicellular organisms establish, organize and achieve coordination persists as a central question in natural sciences. Whereas stochastic gene or protein expressions have clearly demonstrated their role in cellular heterogeneity and are widely studied, the role of cell heterogeneity in the organization of multicellular organisms has been less interrogated. Addressing this question requires adequate tools that quantitatively study ensembles of cells individually rather than group of cells. 

 

My research aims at addressing cell heterogeneity in dynamical and complex systems of cells using the hematopoietic system as a study model. Strikingly hematopoietic cells (immune cells, platelets and red blood cells) compose over 90% of total human cells and correspond to approximately ten trillions of cells (Sender R, 2016). More importantly they all originate from the same cells, the hematopoietic stem cells (HSC), through a process called hematopoiesis. In addition, as immune and blood cells have a short life span (from hours to months) and can response to perturbations like infections, this process is highly dynamical. It is therefore an interesting and challenging model to study how robust cell production is achieved in a complex system at the single cell level.

 

For this purpose, we combine different experimental and mathematical/computational approaches of single cell tracing to study hematopoiesis in vivo. Cellular barcoding is one of these lineage tracing approaches that allow to simultaneously traces the in vivo differentiation of individual cells, allowing to reconstitute the relationship between cell lineages with single cell resolution. In this seminar, I will discuss some of our recent results using cellular barcoding.

 

 

Related Research Questions

 

  1. How do (immune) cells undergo differentiation? How is robust production of cells generated?
  2. What are the steps of differentiation? When is the fate decision made? Does it involve intrinsic, extrinsic or stochastic mechanism?

 

Suggested Reading

 

Cellular barcoding: a technical appraisal.
Naik SH ,Schumacher TN , Perié L.
Exp Hematol. 
2014 Aug;42(8):598-608. doi: 10.1016/j.exphem.2014.05.003. Epub 2014 Jul 1.

 

Retracing the in vivo haematopoietic tree using single-cell methods.
Perié L, Duffy KR.
FEBS Lett. 
2016 Nov;590(22):4068-4083. doi: 10.1002/1873-3468.12299. Epub 2016 Jul 26.

 

 

 

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